A potent antitumour enzyme savitri, neeta asthana and wamik azrni department of biotechnology, himachal pradesh university, summerhill, shimla 171 005, india received 3 may 2002. Since the 1960s it has been known that some leukemic cells are deficient in asparagine synthetase and therefore cannot manufacture sufficient quantities of this essential amino acid to maintain cell viability. Microscopic examination of histological sections of lymph nodes from a canine case of malignant lymphoma at 4 h after treatment with lasparaginase revealed massive destruction of neoplastic cells by what was consistent with apoptosis morphologically. Since the active component of pegaspargase is lasparaginase, the same drugdrug interactions reported with l. Asparaginase asparaginase erwinia chrysanthemi contains an asparagine specific enzyme derived from erwinia chrysanthemi.
Read more about the prescription drug asparaginase injection. Consumer information about the medication asparaginase injection elspar, includes side effects, drug interactions, recommended dosages, and storage information. Apoptosis as the mode of cell death after asparaginase treatment was confirmed in a mouse lymphoma cell line lyth by the. Asparaginase is contraindicated in patients with a history of an allergy to asparaginase, or past or present pancreatitis. In food manufacturing it is used to decrease acrylamide. Lasparaginase is modified by covalently conjugating units of monomethoxypolyethylene glycol peg, which has a molecular weight of 5000, to the enzyme, forming the active ingredient peg lasparaginase 01 19. Schwartz from the department of microbiology, new york university school of. Lasparaginase treatment in acute lymphoblastic leukemia.
Lgm pharma offers this active ingredient but not the finished dosage forms. Lasparaginase definition of lasparaginase by merriam. Serious allergic reactions can occur in patients receiving elspar. Elspar activity is expressed in terms of international units according to the recommendation of the international union of biochemistry. Currently, the enzyme is obtained from bacteria, escherichia coli and erwinia. The promoter of the gene encoding lasparaginase ii, ansb, is coactivated by camp receptor protein crp and the fnr protein scott et al.
History of serious hypersensitivity reactions, including anaphylaxis, to oncaspar or to any of the excipients. Spectrila was designated as an orphan medicinal product on 26 january 2005. You might have it as a treatment for acute lymphoblastic leukaemia all. Purification and characterization of lasparaginase with. Webmd provides common contraindications for asparaginase injection. During the period following lasparaginase protein inhibition, there is a period of increased dna synthesis that leads to increased sensitivity to methotrexate. Rizzari c, zucchetti m, conter v, diomede l, bruno a, gavazzi l, et al. Peglasparaginase pegaspargase chemotherapy side effects, how its given, how it works, precautions and self care tips for treatment of acute lymphocytic leukemia and nonhodgkins lymphoma.
Find patient medical information for asparaginase injection on webmd including its uses, side effects and safety, interactions, pictures, warnings and user ratings. Disclaimer the sds samples above are collected from the internet by chemblink and displayed here for the tutorial purpose only. Where sigma product or stock numbers are specified, equivalent reagents may be substituted. A lasparaginase pode ser irritante aos olhos, a pele e ao trato respiratorio. Lasparaginase gene a therapeutic approach towards drugs. Asparaginase is an enzyme that catalyzes the hydrolysis of l asparagine to l aspartic acid and ammonia. A promising enzyme for treatment of acute lymphoblastic leukiemia richa jain, k. Guidelines for asparaginase administration asparaginase is an antineoplastic agent used most effectively and commonly in pediatrics in the treatment of acute lymphocytic leukemia all, lymphoblastic lymphoma, and acute. Discovery and development as a tumorinhibitory agent, cancer treat rep 65, 111, 1981. Recombinant lasparaginase 1 from saccharomyces cerevisiae. The recommended dose of oncaspar is 2,500 international unitsm. Lasparagine depletion and lasparaginase activity in children with acute lymphoblastic leukemia receiving i.
If asparaginase caused bleeding, a blood clot, or an irritated pancreas before. Oncaspar pegaspargase is indicated as a component of a multiagent chemotherapeutic regimen for the treatment of pediatric and adult patients with. Oncaspar pegaspargase dose, indications, adverse effects. Inquire about dmf, cgmp, price, availability, samples, sourcing, purity and more. Crp is activated in response to carbon sources, and the fnr protein, the fnr gene product, in response to anaerobiosis. Lasparaginase is a high molecular weight enzyme which often induces an immune response in a signi. Lymphoma 6c3hed cells cultured in a medium devoid of lasparaginase lose their susceptibliitity to the effects of guinea pig serum in vivo, j exp med 118, 121, 1963 broome, j lasparaginase. Asparaginase injection elspar side effects, medical. Asparaginase is an enzyme which acts by breaking down lasparagine to aspartic acid and ammonia, this disrupts protein synthesis of tumour cells. Kumar jha, divya pasrija, rati kumari sinha, hare ram singh, vinod kumar nigam and ambrish sharan vidyarthi.
Optimal therapeutic effects depend on a complete and sustained. Department of biotechnology, bit mesra, ranchi, jharkhand, india. Asparaginase hydrolyzes lasparagine to laspartic acid and ammonia in leukemic cells, resulting in the depletion of asparagine, inhibition of protein synthesis, cell cycle arrest in the g1 phase, and apoptosis in susceptible leukemic cell populations. On 19 november 2015, the committee for medicinal products for human use chmp adopted a positive opinion, recommending the granting of a marketing authorisation for the medicinal product spectrila, intended for the treatment of acute lymphoblastic leukaemia. History of serious thrombosis with prior l asparaginase therapy. Lasparaginase is an enzyme that depletes lasparagine an important nutrient for cancer cells resulting in cancertumor cell starvation. Serious thrombotic events, including sagittal sinus thrombosis and pulmonary embolism have been reported with both e.
Lasparaginase and peg asparaginase past, present, and. This characteristic allows lasparaginase to be widely used in therapeutic field, food industry also in biosensor development. Lasparaginase was carried at different temperatures 16, 20, 30, 45 and 50 c. Asparaginase definition of asparaginase by medical. All three preparations deliver a bacterially derived enzyme, lasparaginase, which results in the depletion of plasma asparagine through the hydrolysis of asparagine into. Highlights of prescribing information these highlights do. Dailymed oncaspar pegaspargase injection, solution for. Lasparaginase, commonly used in combination chemotherapy in the treatment of acute lymphoblastic leukemia, has been associated with hemorrhagic and thrombotic cerebrovascular events. Catalyzes hydrolysis of asparagine to aspartic acid and ammonia, thus depleting extracellular supply of an amino acid essential to synthesis of dna and other nucleoproteins. L asparaginase is an enzyme which hydrolyses asparagine. Elspar asparaginase contains the enzyme lasparagine amidohydrolase, type ec2, derived from escherichia coli. University, lodhipur rajpoot delhi road moradabad, uttar. There are two forms of hypersensitivity seen in clinical. Find out what health conditions may be a health risk when taken with asparaginase injection.
As a medication, lasparaginase is used to treat acute lymphoblastic leukemia all, acute myeloid leukemia aml, and nonhodgkins lymphoma. Asparaginase is an enzyme that is used as a medication and in food manufacturing. Asparaginase is widely used in chemotherapeutic regimens for the treatment of acute lymphoblastic leukemia all and has led to a substantial improvement in cure rates, especially in children. Important sources and medicinal applications of lasparaginase hassan mustafa arif, zahid hussain institute of industrial biotechnology, gc university, lahore, pakistan. Sds of lasparaginase, safety data sheets, cas 9015683. The activity of asparaginase is expressed in terms of international units. Lasparaginase is an antitumor agent derived from li. Lasparaginase definition is an enzyme that breaks down the physiologically commoner form of asparagine, is obtained especially from bacteria, and is used especially to treat leukemia. A laboratory derived test to assess lasparaginase levels in the clinical samples. Influence of temperature on deglycosylation deglycosylation of ovalbumin and igy using cladosporium sp. As a result, hypersensitivity to lasparaginase is a clinically relevant issue regardless of the source from which the drug was prepared. Gibson, in pharmacology and therapeutics for dentistry seventh edition, 2017. Lasparaginase is a tetrameric enzyme consisting of four identical subunits, each having a molecular weight of about 35 kda.
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